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n65a mutant gene  (GenScript corporation)

 
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    Structured Review

    GenScript corporation n65a mutant gene
    a , The FimH-binding high-mannose glycan attached to UMOD N275 is located in the groove between the β-hairpin and D10C domain moieties of the protein’s decoy module (left panel). Although this sequon is not conserved in the decoy module of GP2, the groove of the latter contains a different, but closely spaced, N-glycosylation site at position 65 (right panel). b , SEC analysis of the material eluted after incubating an E. coli periplasmic extract containing untagged FimH L with wild-type or <t>N65A</t> mutant GP2 decoy modules immobilized on IMAC beads (left panels). Reducing SDS-PAGE analysis of the corresponding peak fractions (right panels) shows that FimH L binds to the wild-type GP2 decoy module but not to the N65A mutant. n = 2.
    N65a Mutant Gene, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/n65a mutant gene/product/GenScript corporation
    Average 90 stars, based on 1 article reviews
    n65a mutant gene - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Structure of the decoy module of human glycoprotein 2 and uromodulin and its interaction with bacterial adhesin FimH"

    Article Title: Structure of the decoy module of human glycoprotein 2 and uromodulin and its interaction with bacterial adhesin FimH

    Journal: Nature Structural & Molecular Biology

    doi: 10.1038/s41594-022-00729-3

    a , The FimH-binding high-mannose glycan attached to UMOD N275 is located in the groove between the β-hairpin and D10C domain moieties of the protein’s decoy module (left panel). Although this sequon is not conserved in the decoy module of GP2, the groove of the latter contains a different, but closely spaced, N-glycosylation site at position 65 (right panel). b , SEC analysis of the material eluted after incubating an E. coli periplasmic extract containing untagged FimH L with wild-type or N65A mutant GP2 decoy modules immobilized on IMAC beads (left panels). Reducing SDS-PAGE analysis of the corresponding peak fractions (right panels) shows that FimH L binds to the wild-type GP2 decoy module but not to the N65A mutant. n = 2.
    Figure Legend Snippet: a , The FimH-binding high-mannose glycan attached to UMOD N275 is located in the groove between the β-hairpin and D10C domain moieties of the protein’s decoy module (left panel). Although this sequon is not conserved in the decoy module of GP2, the groove of the latter contains a different, but closely spaced, N-glycosylation site at position 65 (right panel). b , SEC analysis of the material eluted after incubating an E. coli periplasmic extract containing untagged FimH L with wild-type or N65A mutant GP2 decoy modules immobilized on IMAC beads (left panels). Reducing SDS-PAGE analysis of the corresponding peak fractions (right panels) shows that FimH L binds to the wild-type GP2 decoy module but not to the N65A mutant. n = 2.

    Techniques Used: Binding Assay, Glycoproteomics, Mutagenesis, SDS Page



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    n65a mutant gene  (GenScript corporation)
    90
    GenScript corporation n65a mutant gene
    a , The FimH-binding high-mannose glycan attached to UMOD N275 is located in the groove between the β-hairpin and D10C domain moieties of the protein’s decoy module (left panel). Although this sequon is not conserved in the decoy module of GP2, the groove of the latter contains a different, but closely spaced, N-glycosylation site at position 65 (right panel). b , SEC analysis of the material eluted after incubating an E. coli periplasmic extract containing untagged FimH L with wild-type or <t>N65A</t> mutant GP2 decoy modules immobilized on IMAC beads (left panels). Reducing SDS-PAGE analysis of the corresponding peak fractions (right panels) shows that FimH L binds to the wild-type GP2 decoy module but not to the N65A mutant. n = 2.
    N65a Mutant Gene, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/n65a mutant gene/product/GenScript corporation
    Average 90 stars, based on 1 article reviews
    n65a mutant gene - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    a , The FimH-binding high-mannose glycan attached to UMOD N275 is located in the groove between the β-hairpin and D10C domain moieties of the protein’s decoy module (left panel). Although this sequon is not conserved in the decoy module of GP2, the groove of the latter contains a different, but closely spaced, N-glycosylation site at position 65 (right panel). b , SEC analysis of the material eluted after incubating an E. coli periplasmic extract containing untagged FimH L with wild-type or N65A mutant GP2 decoy modules immobilized on IMAC beads (left panels). Reducing SDS-PAGE analysis of the corresponding peak fractions (right panels) shows that FimH L binds to the wild-type GP2 decoy module but not to the N65A mutant. n = 2.

    Journal: Nature Structural & Molecular Biology

    Article Title: Structure of the decoy module of human glycoprotein 2 and uromodulin and its interaction with bacterial adhesin FimH

    doi: 10.1038/s41594-022-00729-3

    Figure Lengend Snippet: a , The FimH-binding high-mannose glycan attached to UMOD N275 is located in the groove between the β-hairpin and D10C domain moieties of the protein’s decoy module (left panel). Although this sequon is not conserved in the decoy module of GP2, the groove of the latter contains a different, but closely spaced, N-glycosylation site at position 65 (right panel). b , SEC analysis of the material eluted after incubating an E. coli periplasmic extract containing untagged FimH L with wild-type or N65A mutant GP2 decoy modules immobilized on IMAC beads (left panels). Reducing SDS-PAGE analysis of the corresponding peak fractions (right panels) shows that FimH L binds to the wild-type GP2 decoy module but not to the N65A mutant. n = 2.

    Article Snippet: A corresponding gene and an equivalent UMOD construct, as well as GP2 Δ31-59, Δ31-88 and N65A mutant genes, were also synthesized (GenScript) and all constructs were cloned into pLJ6, a mammalian expression vector derived from pHLsec3 (ref. ).

    Techniques: Binding Assay, Glycoproteomics, Mutagenesis, SDS Page